DDX11 Antibody (D-2) recommended for detection of DDX11 of human origin by WB, IP, IF and ELISA

DDX11 Antibody (D-2): sc-271711

DDX11 Antibody (D-2) is rated 5.0 out of 5 by 3.
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Datasheets

    • DDX11 Antibody (D-2) is a mouse monoclonal IgG2b κ DDX11 antibody, cited in 6 publications, provided at 200 µg/ml
    • raised against amino acids 405-704 mapping within an internal region of DDX11 of human origin
    • DDX11 Antibody (D-2) is recommended for detection of DDX11 of human origin by WB, IP, IF and ELISA
    • Anti-DDX11 Antibody (D-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of DDX11 (D-2): sc-271711.
    • m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DDX11 Antibody (D-2) for WB applications. These reagents are now offered in bundles with DDX11 Antibody (D-2) (see ordering information below).
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What is the recommended fixation method for immunofluorescence staining with DDX11 (D-2): sc-271711 monoclonal antibody?

Asked by: Randy McDonald
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Good specificity in WB in HeLa whole cell lysate Good specificity in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-06-16
Rated 5 out of 5 by from Great immunofluorescence nucleolar and nuclear Great immunofluorescence nucleolar and nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-27
Rated 5 out of 5 by from Robust signal in WB in ES Robust signal in WB in ES-2 whole cell lysate. -SCBT QC
Date published: 2015-04-20
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