DAZL Antibody (E-6)recommended for detection of DAZL of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

DAZL Antibody (E-6): sc-390929

DAZL Antibody (E-6) is rated 4.4 out of 5 by 5.
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Datasheets

    • DAZL Antibody (E-6) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 206-295 mapping at the C-terminus of DAZL of human origin
    • DAZL Antibody (E-6) is recommended for detection of DAZL of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • DAZL Antibody (E-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of DAZL (E-6): sc-390929.
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I am using DAZL (E-6): sc-390929 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from not bad I was not sure whether this antibody apllied to my Species(monkey).,the result was not really ideal.
Date published: 2017-10-10
Rated 5 out of 5 by from GREAT FEATURE Stripe is very clear, can determine the purpose of stripe ,but the specificity is not strong,
Date published: 2017-09-25
Rated 3 out of 5 by from testis I use this antibody these days, but the result of immunohistochemistry is not very well. I will try it again.
Date published: 2017-01-12
Rated 5 out of 5 by from Produced superb immunoperoxidase nuclear Produced superb immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human testis tissue. -SCBT QC
Date published: 2015-02-17
Rated 5 out of 5 by from Satisfactory Western Blot data of DAZL expression Satisfactory Western Blot data of DAZL expression in human testis and mouse testis tissue extracts. -SCBT QC
Date published: 2014-05-27
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