Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
D5DR Double Nickase Plasmid (h): sc-404047-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- D5DR Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- D5DR Double Nickase Plasmid (h) and D5DR Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DRD5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: D5DR Antibody (E-12): sc-376088
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
D5DR Double Nickase Plasmid (h) | sc-404047-NIC | 20 µg | $410.00 | |||
D5DR Double Nickase Plasmid (h2) | sc-404047-NIC-2 | 20 µg | $410.00 |
DRD5 encodes the dopamine receptor D5 (D5DR), a G protein-coupled receptor that predominantly couples to Gs/olf to stimulate adenylyl cyclase, elevate cAMP, and activate PKA-dependent signaling. D5DR signaling intersects with dopamine-regulated synaptic transmission, neuronal excitability, and modulation of downstream pathways including CREB-mediated transcriptional programs. In brain and peripheral tissues, DRD5 contributes to regulation of neurocircuit function and vascular/renal homeostasis through cAMP signaling and receptor cross-talk with other GPCR and ion channel pathways. Dysregulated dopaminergic signaling involving DRD5 has been investigated in the context of neuropsychiatric phenotypes and blood pressure regulation, making it a useful target for mechanistic studies of dopamine pathway biology.
D5DR Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DRD5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DRD5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DRD5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DRD5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.