D-Fructose-1,6-bisphosphate, a common metabolic sugar, is the precursor of glyceraldehyde 3-phosphate and dihydroxyacetone phosphate in the glycolytic pathway. D-Fructose 1,6-bisphosphate trisodium salt octahydrate is an activator of PKLR and PKM2. D-Fructose 1,6-bisphosphate trisodium salt octahydrate is also a substrate of Aldolase, liver FBPaseand and muscle FBPase. It is an allosteric activator of enzymes such as pyruvate kinase and a substrate used to identify and characterize enzymes such as fructose-1,6-bisphosphate aldolase(s) and fructose-1, 6-bisphosphatase(s). D-Fructose-1,6-bisphosphate is studied as a neuroprotective agent in brain injury.