Cytokeratin 8 Antibody (35bH11)recommended for detection of Cytokeratin 8 of human origin by WB, IP, IF and IHC(P)

Cytokeratin 8 Antibody (35bH11): sc-58737

Cytokeratin 8 Antibody (35bH11) is rated 4.8 out of 5 by 6.
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Datasheets

    • Cytokeratin 8 Antibody (35bH11) is a mouse monoclonal IgM (kappa light chain) provided at 200 µg/ml
    • raised against cytoskeletal extract of Hep3B hepatocellular carcinoma cell line of human origin
    • recommended for detection of Cytokeratin 8 of human origin by WB, IP, IF and IHC(P)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See Cytokeratin 8 (C51): sc-8020 for Cytokeratin 8 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cytokeratin 8 (35bH11): sc-58737.
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这种抗体可以鉴定鸡的上皮细胞吗

Asked by: 1234ll
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website. 感谢您的提问。请将您的目的蛋白序列发送到我们的技术支持部门。我们将对比您的序列以及我们的数据,以解答您物种反应性的相关问题。亚洲技术支持电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”。
Answered by: Technical Support 9
Date published: 2019-10-08

Can Cytokeratin 8 (35bH11): sc-58737 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, Cytokeratin 8 (35bH11): sc-58737 monoclonal antibody has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 7
Date published: 2017-02-04
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Rated 5 out of 5 by from Beautiful results in IHC(P) The 35bH11 clone yields beautiful results for Cytokeratin 8 in formalin-fixed paraffin-embedded human gall bladder tissue.
Date published: 2017-01-18
Rated 5 out of 5 by from Single strong band at correct mw in A Single strong band at correct mw in A-43. -SCBT QC
Date published: 2015-03-16
Rated 4 out of 5 by from Good specificity and signal strength with Good specificity and signal strength with some background bands in HeLa, LS1034 and A549 whole cell lysates. -SCBT QC
Date published: 2015-03-06
Rated 5 out of 5 by from Great staining with paraffin Great staining with paraffin-embedded human gall bladder -SCBT QC
Date published: 2015-02-01
Rated 5 out of 5 by from Works well for IF applications Works well for IF applications, positive in HeLa. -SCBT QC
Date published: 2015-01-31
Rated 5 out of 5 by from Band at correct mw in MCF7 Band at correct mw in MCF7. -SCBT QC
Date published: 2013-09-14
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