Cytokeratin 8 Antibody (1E8)recommended for detection of of human origin by WB, IP, IF and IHC(P)

Cytokeratin 8 Antibody (1E8): sc-58736

Cytokeratin 8 Antibody (1E8) is rated 4.6 out of 5 by 5.
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Datasheets

    • Cytokeratin 8 Antibody (1E8) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against C-terminal amino acids 472-483 of Cytokeratin 8 of human origin
    • recommended for detection of of human origin by WB, IP, IF and IHC(P); also reactive with additional species, including bovine
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See Cytokeratin 8 (C51): sc-8020 for Cytokeratin 8 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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Can Cytokeratin 8 (1E8): sc-58736 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: TinTin
Thank you for your question. Yes, Cytokeratin 8 (1E8): sc-58736 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 3 out of 5 by from Bands observed at expected mw in HeLa Bands observed at expected mw in HeLa, A-43 and NCI-H1688 under reducing and under non-reducing conditions. -SCBT QC
Date published: 2015-04-07
Rated 5 out of 5 by from Robust cytoskeletal staining in paraffin Robust cytoskeletal staining in paraffin embedded human prostate. -SCBT QC
Date published: 2015-03-06
Rated 5 out of 5 by from Works well for IF applications Works well for IF applications, cytoskeletal staining in MeOH fixed HeLa cells. -SCBT QC
Date published: 2015-01-30
Rated 5 out of 5 by from Great monoclonal antibody Great monoclonal antibody, works perfect for WB with MCF7. -SCBT QC
Date published: 2015-01-05
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