Cytokeratin 8 Antibody (1E8-C6-B4)recommended for detection of Cytokeratin 8 of human origin by WB, IP and IF

Cytokeratin 8 Antibody (1E8-C6-B4): sc-73480

Cytokeratin 8 Antibody (1E8-C6-B4) is rated 4.0 out of 5 by 3.
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Datasheets

    • Cytokeratin 8 Antibody (1E8-C6-B4) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against C-terminal amino acids 472-483 of Cytokeratin 8 of human origin
    • recommended for detection of Cytokeratin 8 of human origin by WB, IP and IF; also reactive with additional species, including bovine
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See Cytokeratin 8 (C51): sc-8020 for Cytokeratin 8 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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What is the recommended fixation method for immunofluorescence staining with Cytokeratin 8 (1E8-C6-B4): sc-73480 monoclonal antibody?

Asked by: jerojero
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Single clean band in A Single clean band in A-431 cells. -SCBT QC
Date published: 2015-02-15
Rated 4 out of 5 by from Good IF data with methanol Good IF data with methanol-fixed HeLa samples. -SCBT QC
Date published: 2015-01-25
Rated 4 out of 5 by from Good WB data obtained using MCF7 whole cell Good WB data obtained using MCF7 whole cell lysates. -SCBT QC
Date published: 2015-01-23
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