Cytokeratin 5/8 Antibody (RCK102)recommended for detection of Cytokeratin 5 and Cytokeratin 8 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

Cytokeratin 5/8 Antibody (RCK102): sc-32328

Cytokeratin 5/8 Antibody (RCK102) is rated 5.0 out of 5 by 5.
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Datasheets

    • Cytokeratin 5/8 Antibody (RCK102) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against the human lung cancer cell line, MR21
    • recommended for detection of Cytokeratin 5 and Cytokeratin 8 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM; may cross-react with Cytokeratin 18
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See pan-Cytokeratin (C11): sc-8018 for Cytokeratin 5/8 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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How can Cytokeratin 5/8 (RCK102): sc-32328 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Germaine
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-32328 PE) or fluoroscein (sc-32328 FITC).
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Great IHC data with paraffin Great IHC data with paraffin-embedded human colon tumor samples. -SCBT QC
Date published: 2015-04-06
Rated 5 out of 5 by from WB: Strong band in HepG2 samples WB: Strong band in HepG2 samples. -SCBT QC
Date published: 2015-04-01
Rated 5 out of 5 by from WB: Single strong band in T24 whole cell lysates WB: Single strong band in T24 whole cell lysates. -SCBT QC
Date published: 2015-03-19
Rated 5 out of 5 by from Worked great for Flow Cytometry on HeLa human Worked great for Flow Cytometry on HeLa human samples. -SCBT QC
Date published: 2015-02-14
Rated 5 out of 5 by from Good IF data with methanol Good IF data with methanol-fixed HeLa samples. -SCBT QC
Date published: 2015-02-12
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