Cytokeratin 5/8 Antibody (C50)recommended for detection of Cytokeratin 5/8 of human origin by WB, IP, IF, IHC(P), FCM and ELISA

Cytokeratin 5/8 Antibody (C50): sc-8021

Cytokeratin 5/8 Antibody (C50) is rated 5.0 out of 5 by 4.
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Datasheets

    • Cytokeratin 5/8 Antibody (C50) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against cytoskeleton preparation of HeLa cells
    • recommended for detection of Cytokeratin 5/8 of human origin by WB, IP, IF, IHC(P), FCM and ELISA; may cross-react with Cytokeratin 18
    • available conjugated to either phycoerythrin, Alexa Fluor® 488 or Alexa Fluor® 647 for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 405 for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See pan-Cytokeratin (C11): sc-8018 for Cytokeratin 5/8 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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Can Cytokeratin 5/8 (C50): sc-8021 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Cytokeratin 5/8 (C50): sc-8021 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Great results in WB with Cu 145 Great results in WB with Cu 145, TSUPr1, LNCaP, C4-2, and PC-3 cell lysates -SCBT Publication Review
Date published: 2015-01-28
Rated 5 out of 5 by from Works well with paraffin Works well with paraffin-embedded sections in IHC -SCBT QC
Date published: 2015-01-19
Rated 5 out of 5 by from Good staining with human colorectal cancer Good staining with human colorectal cancer and colonic mucosa samples in IHC -SCBT Publication Review
Date published: 2015-01-08
Rated 5 out of 5 by from Directly conjugated form offers beautiful Directly conjugated form offers beautiful resolution of cytoskeletal structure in IF -SCBT QC
Date published: 2014-01-28
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