Cytokeratin 5/8 Antibody (5F295)recommended for detection of Cytokeratin 5/8 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

Cytokeratin 5/8 Antibody (5F295): sc-70928

Cytokeratin 5/8 Antibody (5F295) is rated 4.0 out of 5 by 3.
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Datasheets

    • Cytokeratin 5/8 Antibody (5F295) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against the human lung cancer cell line, MR21
    • recommended for detection of Cytokeratin 5/8 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM; may cross-react with Cytokeratin 18
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See pan-Cytokeratin (C11): sc-8018 for Cytokeratin 5/8 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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What is the recommended starting dilution for Western blot for sc-70928: Cytokeratin 5/8 (5F295) monoclonal antibody?

Asked by: Germaine
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Great WB data in HepG2 samples Great WB data in HepG2 samples. -SCBT QC
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