Anti-CYP1A2 Antibody (3B8C1) recommended for detection of CYP1A2 of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-CYP1A2 Antibody (3B8C1): sc-53614

Anti-CYP1A2 Antibody (3B8C1) is rated 3.8 out of 5 by 4.
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Datasheets

    • Anti-CYP1A2 Antibody (3B8C1) is a mouse monoclonal IgG1 κ CYP1A2 antibody, cited in 27 publications, provided at 200 µg/ml
    • raised against cytochrome P450 protein of rat origin
    • Anti-CYP1A2 Antibody (3B8C1) is recommended for detection of CYP1A2 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • Anti-CYP1A2 Antibody (3B8C1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CYP1A2 (3B8C1): sc-53614.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CYP1A2 Antibody (3B8C1) for WB and IHC(P) applications. These reagents are now offered in bundles with CYP1A2 Antibody (3B8C1) (see ordering information below).
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re: CYP1A2 (3B8C1): sc-53614 - What was the precise antigen used to prepare the MnAb? What does the MnAb cross-react with?

Asked by: hlll
Thank you for your question. Unfortunately, we do not have any precise information about which antigen was used to develop this monoclonal antibody. It was raised against cytochrome P450 protein of rat origin, but the exact antigen sequence is not known. This antibody is recommended for detection of CYP1A2 of mouse, rat and human origin.
Answered by: Technical Support
Date published: 2019-01-26

I am using CYP1A2 (3B8C1): sc-53614 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Dr Ninau Qelp
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from promising IHC staining I got a promising IHC staining result with DAB in mouse liver.
Date published: 2019-11-22
Rated 4 out of 5 by from Cytoplasmic staining observed in hepatocytes Cytoplasmic staining observed in hepatocytes by immunoperoxidase staining of formalin fixed, paraffin embedded (FFPE) human liver tissue. -SCBT QC
Date published: 2015-04-27
Rated 4 out of 5 by from Cytoplasmic localization of the CYP1A2 protein Cytoplasmic localization of the CYP1A2 protein demonstrated in the immunoperoxidase staining of hepatocytes and bile duct cells of formalin fixed, paraffin embedded (FFPE) human liver tissue. -SCBT QC
Date published: 2015-03-17
Rated 4 out of 5 by from Nice band observed via Western Blot using Nice band observed via Western Blot using human liver extract. -SCBT QC
Date published: 2013-03-07
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