Anti-CYP1A1 Antibody (A-9) recommended for detection of CYP1A1 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-CYP1A1 Antibody (A-9): sc-393979

Anti-CYP1A1 Antibody (A-9) is rated 4.3 out of 5 by 7.
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Datasheets

    • Anti-CYP1A1 Antibody (A-9) is a mouse monoclonal IgG1 κ CYP1A1 antibody, cited in 16 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 479-506 near the C-terminus of CYP1A1 of mouse origin
    • Anti-CYP1A1 Antibody (A-9) is recommended for detection of CYP1A1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-CYP1A1 Antibody (A-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CYP1A1 (A-9): sc-393979.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CYP1A1 Antibody (A-9) for WB applications. These reagents are now offered in bundles with CYP1A1 Antibody (A-9) (see ordering information below).
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HI TECHNICAL SUPPORT, CYP1A1 (A-9) in my phd progect. i found clear band for mda-mb468 on nitrocelluse membrane at dilution 1:100 incubation over night - i could not detect band in MCF-7 and mda-231 could you help me please as i want to purchas

Asked by: deem
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-05-20

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 12
Date published: 2017-01-13

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 12
Date published: 2017-01-13
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Rated 4 out of 5 by from Worked well for human and mouse samples Worked well when using for WB at 1:200 dilution. Provided nice clear bands in both human and mouse tissue lysates after longer exposure times.
Date published: 2018-03-22
Rated 1 out of 5 by from Not really working.. :-( Tried in WB of human hepatocytes Huh7 and Huh7.5. No bands at the expected size, only some faint bands at the bottom of the blot (maybe 25-30kDa).
Date published: 2018-01-22
Rated 5 out of 5 by from Good for WB 1:200 diluted for wb of rat heart protein,there is a clear band at the position above 70KD.
Date published: 2017-10-20
Rated 5 out of 5 by from Nice Western Blot Nice Western Blot data of CYP1A1 expression in HCC whole cell lysates.
Date published: 2017-05-03
Rated 5 out of 5 by from Excellent new antibody! There are several good CYP1A1 clones to choose from, but this new CYP1A1 (A-9) clone gives excellent results in our in-house testing by Western blotting in mouse, rat and human samples.
Date published: 2017-01-30
Rated 5 out of 5 by from Great Great. It appeared as a single band at the size expected. No background bands found. I used HCC827 Lung Cancer Cells for western blot.
Date published: 2015-03-26
Rated 5 out of 5 by from Great single bands in WB with HeLa and Neuro Great single bands in WB with HeLa and Neuro-2A whole cell lysates -SCBT QC
Date published: 2014-07-13
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