Anti-CYP1A1 Antibody (1A3-03) recommended for detection of CYP1A1 of mouse, rat, human and porcine origin by WB, IP, IF, FCM and ELISA

Anti-CYP1A1 Antibody (1A3-03): sc-101828

Anti-CYP1A1 Antibody (1A3-03) is rated 4.3 out of 5 by 3.
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Datasheets

    • CYP1A1 Antibody (1A3-03) is a mouse monoclonal IgG1 κ, cited in 4 publications, provided at 200 µg/ml
    • raised against CYP1A1 isolated from 3-methylcholanthrene treated microsomes of rat liver origin
    • Anti-CYP1A1 Antibody (1A3-03) is recommended for detection of CYP1A1 of mouse, rat, human and porcine origin by WB, IP, IF, FCM and ELISA; non cross-reactive with CYP1A2
    • Anti-CYP1A1 Antibody (1A3-03) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CYP1A1 (1A3-03): sc-101828.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CYP1A1 Antibody (1A3-03) for WB applications. These reagents are now offered in bundles with CYP1A1 Antibody (1A3-03) (see ordering information below).
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Can CYP1A1 (1A3-03): sc-101828 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Cweed
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CYP1A1 (1A3-03): sc-101828 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Works great! Worked great for human and mouse tissue lysates when used at 1:200.
Date published: 2018-03-23
Rated 5 out of 5 by from positive western blot We used this antibody for a western blot experiment and got positive bands at the correct molecular weight
Date published: 2017-01-18
Rated 3 out of 5 by from Worked with human transfected lysate by WB Worked with human transfected lysate by WB -SCBT QC
Date published: 2014-11-24
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