Anti-CYP17A1 Antibody (D-12) recommended for detection of CYP17A1 of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-CYP17A1 Antibody (D-12): sc-374244

Anti-CYP17A1 Antibody (D-12) is rated 4.2 out of 5 by 6.
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Datasheets

    • Anti-CYP17A1 Antibody (D-12) is a mouse monoclonal IgG2a κ CYP17A1 antibody, cited in 11 publications, provided at 200 µg/ml
    • raised against amino acids 461-508 mapping at the C-terminus of CYP17A1 of human origin
    • CYP17A1 Antibody (D-12) is recommended for detection of CYP17A1 of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-CYP17A1 Antibody (D-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CYP17A1 (D-12): sc-374244.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for CYP17A1 Antibody (D-12) for WB and IHC(P) applications. These reagents are now offered in bundles with CYP17A1 Antibody (D-12) (see ordering information below).
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I am using CYP17A1 (D-12): sc-374244 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: AbPolly
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-23
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Rated 4 out of 5 by from Works very well for WB in the mouse testis The good: We have tried every cyp17a1 antibody we could find (from multiple companies) to identify the best one for WB, IF, and IHC. This antibody is by far the best for WB for use in the mouse testis at 1:250 dilution. There are two non-specific bands, but they are very obviously the wrong size. All other antibodies we tried for WB gave many more non-specific bands. We have tried three different lots of this antibody and they have all worked similarly! The not so good: 1) This antibody did not work well for IF/IHC in the mouse testis using our standard protocol. There was a lot of non-specific binding in cell types that do not express this enzyme. Perhaps with more optimization on our end this could be improved. 2) This antibody seems to lose potency within a few months despite being stored as directed, and we have to re-order way before we have used the entire amount. Its still worth it though, for WB.
Date published: 2019-08-30
Rated 5 out of 5 by from Great for WB The CYP17A1 Antibody (D-12): sc-374244 works very well for Western blot applications, showing strong and clear band with minimal background.
Date published: 2017-02-08
Rated 1 out of 5 by from Ne fonctionne pas chez la souris Ne fonctionne ni en WB sur des extraits de testicules de souris ni en immunohistochimie sur des testicules de souris
Date published: 2017-02-02
Rated 5 out of 5 by from Worked great with porcine samples! I received a sample of this antibody for use in western blotting. It works great with porcine testis samples! It gave one beautiful band of an appropriate molecular weight. It also demonstrated linearity with increasing protein concentrations and the negative control was clear. We are very happy.
Date published: 2016-11-30
Rated 5 out of 5 by from work with pig testies leydig cells work with pig testies leydig cells
Date published: 2016-08-08
Rated 5 out of 5 by from Worked with human transfected lysate by WB Worked with human transfected lysate by WB -SCBT QC
Date published: 2014-08-25
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