cyclin YL1 Antibody (D-4)recommended for detection of cyclin YL1 of mouse, rat and human origin by WB, IP, IF and ELISA

cyclin YL1 Antibody (D-4): sc-514637

cyclin YL1 Antibody (D-4) is rated 5.0 out of 5 by 2.
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Datasheets

    • cyclin YL1 Antibody (D-4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 3-51 mapping at the N-terminus of cyclin YL1 of human origin
    • cyclin YL1 Antibody (D-4) is recommended for detection of cyclin YL1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • cyclin YL1 Antibody (D-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin YL1 (D-4): sc-514637.
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For Western Blot, is it recommended to use denatured or non-denatured conditions with cyclin YL1 (D-4): sc-514637 monoclonal antibody?

Asked by: Germaine
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from good product Clean strong signal of a single band at ~36 kDa in HeLa whole cell lysate. Strong non-specific band seen at ~52 kDa.
Date published: 2018-04-26
Rated 5 out of 5 by from an excellent antibody for WB This antibody generates a clear and strong signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
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