Anti-cyclin T1 Antibody (E-3) recommended for detection of cyclin T1 of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-cyclin T1 Antibody (E-3): sc-271348

Anti-cyclin T1 Antibody (E-3) is rated 4.9 out of 5 by 9.
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Datasheets

    • Anti-cyclin T1 Antibody (E-3) is a mouse monoclonal IgG2b κ cyclin T1 antibody, cited in 14 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 671-708 near the C-terminus of cyclin T1 of human origin
    • cyclin T1 Antibody (E-3) is recommended for detection of cyclin T1 of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-cyclin T1 Antibody (E-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-271348 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin T1 (E-3): sc-271348.
    • m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for cyclin T1 Antibody (E-3) for WB and IHC(P) applications. These reagents are now offered in bundles with cyclin T1 Antibody (E-3) (see ordering information below).
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What application is the blocking peptide sc-271348 P appropriate for?

Asked by: jerojero
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from good product It is a good antibody with clean background for WB.
Date published: 2018-09-11
Rated 4 out of 5 by from Single band in western blot Used at 1:500 to detect endogenous protein in 30ug nuclear extract from HEK293T.
Date published: 2018-08-02
Rated 5 out of 5 by from Great for WB This antibody is good for Western blot with human cell nuclear extract.
Date published: 2018-01-13
Rated 5 out of 5 by from Great for western blot and work for IP as well Great for western blot and work for IP as well. We used it for MCF-7 cell lysate.
Date published: 2017-11-30
Rated 5 out of 5 by from a fantastic antibody for WB This antibody generates a clear and very strong signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 5 out of 5 by from clean band This antibody works well in western bloting experiments.The band is clean although the antibody titer is not high enough.
Date published: 2017-08-18
Rated 5 out of 5 by from Beautiful signal this antibody works very well for western blotting; really clean Cyclin T1 band; I used it on protein extracts from different types of human cells (like HeLa, HCT116,etc). looks great.
Date published: 2017-04-21
Rated 5 out of 5 by from Great antibody for Western Great antibody for Western, strong band detected in K-562 and Jurkat nuclear extracts. -SCBT QC
Date published: 2015-01-09
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