cyclin T1 Antibody (C-6)recommended for detection of cyclin T1 of mouse, rat and human origin by WB, IP, IF and ELISA

cyclin T1 Antibody (C-6): sc-271575

cyclin T1 Antibody (C-6) is rated 5.0 out of 5 by 3.
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Datasheets

    • cyclin T1 Antibody (C-6) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 261-505 mapping within an internal region of cyclin T1 of human origin
    • cyclin T1 Antibody (C-6) is recommended for detection of cyclin T1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • cyclin T1 Antibody (C-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-271575 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin T1 (C-6): sc-271575.
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What is the recommended fixation method for immunofluorescence staining with cyclin T1 (C-6): sc-271575 monoclonal antibody?

Asked by: Professor Griffin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from a good antibody for WB This antibody generates a clear signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 5 out of 5 by from Works very nicely for IF with Hep G2 Works very nicely for IF with Hep G2. -SCBT QC
Date published: 2015-03-13
Rated 5 out of 5 by from Clean blot with single strong band in K562 Clean blot with single strong band in K562. -SCBT QC
Date published: 2014-08-21
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