Anti-cyclin O Antibody (F-3) recommended for detection of cyclin O of human origin by WB, IP, IF and ELISA

Anti-cyclin O Antibody (F-3): sc-374633

Anti-cyclin O Antibody (F-3) is rated 4.0 out of 5 by 2.
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Datasheets

    • Anti-cyclin O Antibody (F-3) is a mouse monoclonal IgG2b (kappa light chain) cyclin O antibody provided at 200 µg/ml
    • raised against amino acids 1-267 mapping at the N-terminal of cyclin O of human origin
    • cyclin O Antibody (F-3) is recommended for detection of cyclin O of human origin by WB, IP, IF and ELISA
    • Anti-cyclin O Antibody (F-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-374633 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin O (F-3): sc-374633.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for cyclin O Antibody (F-3). This work is in progress.
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Hi, I'm just about to begin a project with patient derived mutant CCNO cells. I was interested in the free 10 µL sample offered on your website for the anti-cyclin O antibody sc-374633. Could you please contact me in this regard? Many thanks. Guy.

Asked by: GYealland
Thank you for your question. Please contact technical service directly to arrange for free antibody samples.
Answered by: Tech Support Europe
Date published: 2021-04-28

What is the recommended starting dilution for Western blot for sc-374633: cyclin O (F-3) monoclonal antibody?

Asked by: cjMara
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 4 out of 5 by from a good antibody for WB This antibody generates a clear signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract), albeit with higher background
Date published: 2017-09-18
Rated 4 out of 5 by from Good specificity and signal strength in human Good specificity and signal strength in human transfected cell lysates.
Date published: 2013-12-27
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