Anti-cyclin L1 Antibody (Q-1) recommended for detection of cyclin L1 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-cyclin L1 Antibody (Q-1): sc-81843

Anti-cyclin L1 Antibody (Q-1) is rated 4.0 out of 5 by 2.
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Datasheets

    • Anti-cyclin L1 Antibody (Q-1) is a mouse monoclonal IgG1 (kappa light chain) cyclin L1 antibody provided at 100 µg/ml
    • raised against recombinant cyclin L1 of human origin
    • recommended for detection of cyclin L1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for cyclin L1 Antibody (Q-1). This work is in progress.
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I am using cyclin L1 (Q-1): sc-81843 for immunofluorescence, what dilution of this antibody should I use?

Asked by: cjMara
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Worked in IF but somewhat faint staining in HeLa Worked in IF but somewhat faint staining in HeLa. -SCBT QC
Date published: 2015-03-11
Rated 4 out of 5 by from Band at correct mw in transfected lysates Band at correct mw in transfected lysates. -SCBT QC
Date published: 2014-10-14
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