Anti-cyclin J Antibody (2126C1a) recommended for detection of cyclin J of human origin by WB and IP

Anti-cyclin J Antibody (2126C1a): sc-81064

Anti-cyclin J Antibody (2126C1a) is rated 3.0 out of 5 by 3.
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Datasheets

    • Anti-cyclin J Antibody (2126C1a) is a mouse monoclonal IgG1 cyclin J antibody provided at 100 µg/ml
    • raised against a recombinant protein corresponding to a region near the C-terminus of cyclin J of human origin
    • recommended for detection of cyclin J of human origin by WB and IP
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin J (2126C1a): sc-81064.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for cyclin J Antibody (2126C1a). This work is in progress.
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I am using cyclin J (2126C1a): sc-81064 antibody for immunoprecipitation, how much antibody should I use?

Asked by: AbPolly
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 2 out of 5 by from good for recombinant protein detection Tested for HEK293 and HeLa with or without overexpression system. Worked well for detecting overexpressed recombinant protein but not endogenous protein.
Date published: 2018-09-23
Rated 5 out of 5 by from good product Clean but weak signal of a single band at ~38 kDa in HeLa whole cell lysate.
Date published: 2018-04-26
Rated 2 out of 5 by from Works well for WB with recombinant fusion Works well for WB with recombinant fusion protein sample.
Date published: 2015-01-15
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