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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
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Labware ships FedEx Ground free of charge to the contiguous US
Cyclin J is a 372 amino acid protein encoded by the human gene CCNJ located on chromosome 10. Cyclin J belongs to the cyclin family and contains one cyclin N-terminal domain. A cyclin forms a complex with its partner cyclin-dependent kinase (Cdk), which activates the protein kinase function of the Cdk. Cyclins are so named because their concentration varies in a cyclical fashion during the cell cycle. They are produced or degraded as needed in order to drive the cell through the different stages of the cell cycle. When its concentrations in the cell are low, the cyclin detaches from the Cdk, inhibiting the activity of the enzyme, probably by causing a protein chain to block the enzymatic site. Cyclin J-associated kinase activity is required for the early embryonic division cycles.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
I am using cyclin J (2126C1a): sc-81064 antibody for immunoprecipitation, how much antibody should I use?
Asked by: AbPolly
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here:
Rated 2 out of
good for recombinant protein detectionTested for HEK293 and HeLa with or without overexpression system. Worked well for detecting overexpressed recombinant protein but not endogenous protein.
Date published: 2018-09-23
Rated 5 out of
good productClean but weak signal of a single band at ~38 kDa in HeLa whole cell lysate.
Date published: 2018-04-26
Rated 2 out of
Works well for WB with recombinant fusionWorks well for WB with recombinant fusion protein sample.
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