Anti-cyclin G1 Antibody (F-5) recommended for detection of cyclin G1 of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-cyclin G1 Antibody (F-5): sc-8016

Anti-cyclin G1 Antibody (F-5) is rated 4.7 out of 5 by 6.
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Datasheets

    • Anti-cyclin G1 Antibody (F-5) is a mouse monoclonal IgG1 κ cyclin G1 antibody, cited in 11 publications, provided at 200 µg/ml
    • raised against amino acids 1-46 mapping at the N-terminus of cyclin G1 of human origin
    • cyclin G1 Antibody (F-5) is recommended for detection of cyclin G1 of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-cyclin G1 Antibody (F-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin G1 (F-5): sc-8016.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for cyclin G1 Antibody (F-5) for WB and IHC(P) applications. These reagents are now offered in bundles with cyclin G1 Antibody (F-5) (see ordering information below).
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I am using cyclin G1 (F-5): sc-8016 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from WBに適した抗体 Western blottingに用いましたが、特異的でクリアなバンドを見ることができて満足でした。
Date published: 2019-07-31
Rated 4 out of 5 by from ok Weak signal of a single band at ~37 kDa in HeLa whole cell lysate. Strong non-specific band seen at ~41 kDa.
Date published: 2018-04-26
Rated 4 out of 5 by from a good antibody for WB This antibody generates a clear signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 5 out of 5 by from Good nuclear staining with paraffin Good nuclear staining with paraffin-embedded human colon samples. -SCBT QC
Date published: 2015-03-05
Rated 5 out of 5 by from Works very nicely for IF on human cancer Works very nicely for IF on human cancer cell lines. -SCBT Publication Review
Date published: 2015-01-19
Rated 5 out of 5 by from Clean blot with single strong band at expected Clean blot with single strong band at expected size with recombinant His-tagged cyclin G1. -SCBT QC
Date published: 2014-10-03
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