Anti-cyclin F Antibody (B-6) recommended for detection of cyclin F of human origin by WB, IP, IF and ELISA

Anti-cyclin F Antibody (B-6): sc-515207

Anti-cyclin F Antibody (B-6) is rated 4.0 out of 5 by 3.
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Datasheets

    • Anti-cyclin F Antibody (B-6) is a mouse monoclonal IgG2b κ cyclin F antibody, cited in 2 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 774-798 at the C-terminus of cyclin F of human origin
    • cyclin F Antibody (B-6) is recommended for detection of cyclin F of human origin by WB, IP, IF and ELISA
    • Anti-cyclin F Antibody (B-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin F (B-6): sc-515207.
    • m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for cyclin F Antibody (B-6) for WB applications. These reagents are now offered in bundles with cyclin F Antibody (B-6) (see ordering information below).
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What application is the blocking peptide sc-515207 P appropriate for?

Asked by: Randy McDonald
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 3 out of 5 by from an OK antibody for WB This antibody generates a signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract). However, the signal intensity is rather weak.
Date published: 2017-09-18
Rated 4 out of 5 by from work for wb I get the sample and use it to WB,it can work at 1:500,but the band is poor
Date published: 2017-08-09
Rated 5 out of 5 by from clean but weak signal In HeLa, it has a weak signal, but is clean. Needs to be used at least at 1:250.
Date published: 2017-04-12
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