cyclin F Antibody (2123D1a)recommended for detection of cyclin F of human origin by WB and IP

cyclin F Antibody (2123D1a): sc-81242

cyclin F Antibody (2123D1a) is rated 4.5 out of 5 by 2.
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Datasheets

    • cyclin F Antibody (2123D1a) is a mouse monoclonal IgG1 provided at 100 µg/ml
    • raised against a recombinant protein corresponding to the C-terminal region of cyclin F of human origin
    • recommended for detection of cyclin F of human origin by WB and IP
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I am using cyclin F (2123D1a): sc-81242 antibody for immunoprecipitation, how much antibody should I use?

Asked by: jenni
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from 产品功能很好 效价比很高,抗体浓度1:500,4度孵育过夜就可得到清晰条带,在鼻咽癌和前列腺癌中都可发出清晰条带。
Date published: 2016-11-26
Rated 4 out of 5 by from Antibody works decently in WB with Ramos Antibody works decently in WB with Ramos nuclear extract. -SCBT QC
Date published: 2014-03-02
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