cyclin E Antibody (HE12)recommended for detection of cyclin E (doublet at 50 kDa and single band at 42 kDa) of mouse, rat and human origin by WB, IP, IF and IHC(P)

cyclin E Antibody (HE12): sc-247

cyclin E Antibody (HE12) is rated 4.7 out of 5 by 17.
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Datasheets

    • cyclin E Antibody (HE12) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against recombinant cyclin E of human origin
    • cyclin E Antibody (HE12) is recommended for detection of cyclin E (doublet at 50 kDa and single band at 42 kDa) of mouse, rat and human origin by WB, IP, IF and IHC(P); Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
    • cyclin E Antibody (HE12) is available conjugated to FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 594 for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin E (HE12): sc-247.
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Which buffer to use for western blot? I used 5% milk in TBS-T for blocking and for both primary and secondary Ab and got no band. Thanks

Asked by: Yaelle
Thank you for your question. We recommend using 5% nonfat milk in TBST for blocking and for both primary and secondary antibody incubations. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-03-03

Is the antibody directed against the whole protein or any particular site (c-or n-terminal?)

Asked by: portland
Thank you for your question. This antibody was made against a recombinant synthetic peptide corresponding to Human Cyclin E1. The exact epitope has not been epitope mapped, so I do not have further information regarding any particular site. If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2018-02-07

Can the antibody used on both rat and mouse source samples? How long will it take to get the antibody?

Asked by: Guanwen
Thank you for your question. cyclin E Antibody (HE12): sc-247 is recommended for detection of cyclin E in mouse, rat and human samples. Please contact our Asian Support Office to inquire about current product availability and shipping and delivery times for your institution. Please call our Asian Support office toll-free at 800-820-8626, email asia@scbio.cn, or contact us by live chat.
Answered by: Technical Support
Date published: 2017-04-07

Does it recognize both CCNE1 and CCNE2? thanks!

Asked by: NickNR
Thank you for your question. cyclin E Antibody (HE12): sc-247 is recommended for detection of cyclin E (CCNE1). We have not tested the antibody for reactivity against CCNE2.
Answered by: Technical Support
Date published: 2017-03-30

Is it possible to use this mouse monoclonal antibody to detect cyclin E in canine samples?

Asked by: Trav11
To inquire about additional species reactivity not listed on our website, please contact our Technical Service department at 800-457-3801 and we will be more than happy to run sequence analysis for your desired species.
Answered by: Tech Service 8
Date published: 2016-12-16

Can I use the cyclin E Antibody (HE12) for immunofluorescence if I am working with mouse tissue samples?

Asked by: Trav11
If looking to perform immunofluorescence in mouse tissue samples, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, the cyclin E Antibody (HE12): sc-247 is available conjugated to fluorescein (sc-247 FITC, 200 µg/ml) and TRITC (sc-247 TRITC, 200 µg/ml).
Answered by: Tech Service 8
Date published: 2016-12-16
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Rated 5 out of 5 by from good product It is a good antibody with clean background for WB.
Date published: 2018-09-11
Rated 5 out of 5 by from Very strong signal Sharp and strong band in westen blot carried out on samples derived from human primary macrophages.
Date published: 2018-03-12
Rated 4 out of 5 by from HE12, Santa Cruz: a good antibody This antibody works really well and for this reason we choose it among all the other that we had the possibility to test.
Date published: 2018-01-18
Rated 5 out of 5 by from Good antibody! 1:200 diluted for wb of rat heart protein,the band is strong and special
Date published: 2017-12-30
Rated 5 out of 5 by from a good antibody for WB This antibody generates a clear signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 5 out of 5 by from Strong signal I used cyclin E (HE12) antibody (1:500) dilution to check S phase in cell cycle experiment and got a strong signal and I can see a difference among my samples.
Date published: 2017-08-25
Rated 4 out of 5 by from WB in T47D, BT474 and BT474HR cells 1/500 dilution of the present antibody yielded the expected band in 50ug of T47D, BT474 and BT474HR cells
Date published: 2017-08-22
Rated 5 out of 5 by from Cyclin E Antibody Good antibody with strong signal tested in different cancer cell lines
Date published: 2017-05-04
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