Anti-cyclin E Antibody (HE111) recommended for detection of cyclin E of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-cyclin E Antibody (HE111): sc-248

Anti-cyclin E Antibody (HE111) is rated 4.8 out of 5 by 6.
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Datasheets

    • cyclin E Antibody (HE111) is a mouse monoclonal IgG1 λ, cited in 133 publications, provided at 200 µg/ml
    • raised against recombinant cyclin E of human origin
    • Anti-cyclin E Antibody (HE111) is recommended for detection of cyclin E of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • Anti-cyclin E Antibody (HE111) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to TRITC for IF, IHC(P) and FCM
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-248 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin E (HE111): sc-248.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for cyclin E Antibody (HE111). This work is in progress.
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Can this antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. We recommend following our recommended protocol, provided below, https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 7
Date published: 2017-01-25
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Rated 5 out of 5 by from a good antibody for WB This antibody generates a clear signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 5 out of 5 by from mouse liver nice antibody 1:500 dilution, in mouse liver tissue.
Date published: 2017-03-31
Rated 5 out of 5 by from Great IHC results Tested multiple samples by IHC in paraffin embedded sections with great results!
Date published: 2017-01-17
Rated 5 out of 5 by from Good data with paraffin Good data with paraffin-embedded human ovary tissue. -SCBT Publication Review
Date published: 2015-02-11
Rated 4 out of 5 by from This antibody works well and can be used This antibody works well and can be used for IP with MEG-01 samples. -SCBT QC
Date published: 2015-01-29
Rated 5 out of 5 by from WB: Great antibody WB: Great antibody, good images for publication with human U2OS. -SCBT Publication Review
Date published: 2014-08-20
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