Anti-cyclin E Antibody (E-4) is a mouse monoclonal IgG2b κ cyclin E antibody, cited in 80 publications, provided at 200 µg/ml
specific for an epitope mapping between amino acids 367-396 at the C-terminus of cyclin E of rat origin
Anti-cyclin E Antibody (E-4) is recommended for detection of cyclin E1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
Anti-cyclin E Antibody (E-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin E (E-4): sc-377100.
m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for cyclin E Antibody (E-4) for WB and IHC(P) applications. These reagents are now offered in bundles with cyclin E Antibody (E-4) (see ordering information below).
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cyclin E Antibody (E-4) is a high quality monoclonal cyclin E antibody (also designated CCNE1 antibody) suitable for the detection of the cyclin E protein of mouse, rat and human origin. cyclin E Antibody (E-4) is available as both the non-conjugated anti-cyclin E antibody form, as well as multiple conjugated forms of anti-cyclin E antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner. The cyclin E/E2F complex is detected primarily during the G1 phase of the cell cycle and decreases as cells enter S phase. E2F is known to be a critical transcription factor for expression of several S phase specific proteins.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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