Anti-cyclin E Antibody (E-4) recommended for detection of cyclin E1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-cyclin E Antibody (E-4): sc-377100

Anti-cyclin E Antibody (E-4) is rated 4.4 out of 5 by 34.
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Datasheets

    • Anti-cyclin E Antibody (E-4) is a mouse monoclonal IgG2b κ cyclin E antibody, cited in 80 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 367-396 at the C-terminus of cyclin E of rat origin
    • Anti-cyclin E Antibody (E-4) is recommended for detection of cyclin E1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-cyclin E Antibody (E-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin E (E-4): sc-377100.
    • m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for cyclin E Antibody (E-4) for WB and IHC(P) applications. These reagents are now offered in bundles with cyclin E Antibody (E-4) (see ordering information below).
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What Li-COR/Odyssey compatible secondary is recommended for use with this cyclin E mouse primary?

Asked by: AbPolly
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 9
Date published: 2017-01-18

您好,我想问下:我这个抗体用的并不好,我想给差评可以吗?我看网站上的评论99%都是五星,我就犹豫了,差评了还能收到优惠券吗?

Asked by: xiaomao
感谢您的提问。关于如何提交评论的问题请联系我们的技术支持部门:电话(+86) 021-60936351,邮箱[email protected]。 Thank you for your question. For questions regarding reviews, please contact our Technical Services department at (+86) 021-60936351 or [email protected]
Answered by: Technical Support 12
Date published: 2016-12-22
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Rated 5 out of 5 by from 产品很好 我10月份订购的此抗体,实验室之前的货号停产更换为此货号,在试验后 条带清晰,效果明显,做细胞增殖需要的朋友,很推荐此款抗体!
Date published: 2018-11-26
Rated 5 out of 5 by from Excellent The antibody was used to examine the protein of cyclin E in rat tissues. It is excellent. The bands were clear and specific.
Date published: 2018-11-14
Rated 5 out of 5 by from Great product!! It's a great product for me to analyze Western blotting.
Date published: 2018-04-22
Rated 1 out of 5 by from Unspecific bands We used this antibody in WB, and the bands we got were not at the expected size!
Date published: 2018-03-15
Rated 4 out of 5 by from Satisfying signal Quite sensitive, allows to detect cyclin E in GM-CSF differentiated human macrophages
Date published: 2018-03-12
Rated 4 out of 5 by from IHC(paraffin) works well 1:200 dilution and EDTA antigen retrieval works fine in mouse liver tissue.
Date published: 2018-01-15
Rated 5 out of 5 by from Excellent Antibody! Received a sample antibody of Cyclin E (E-4) very quickly. Strong signal for WB using HF1 cells at 1:500.
Date published: 2017-10-13
Rated 5 out of 5 by from great feature! it's so happy to receive the cyclin E Antibody (E-4) sample a month ago. it worked well in my experiments. The cells i used is hela cells and the dilution ratio is 1:1000 for WB.
Date published: 2017-10-10
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