cyclin E Antibody (B-7)recommended for detection of cyclin E of human origin by WB, IP, IF and IHC(P)

cyclin E Antibody (B-7): sc-48420

cyclin E Antibody (B-7) is rated 4.0 out of 5 by 1.
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Datasheets

    • cyclin E Antibody (B-7) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-145 of cyclin E of human origin
    • recommended for detection of cyclin E of human origin by WB, IP, IF and IHC(P)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See cyclin E (E-4): sc-377100 for cyclin E antibody conjugates, including AC, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647.
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I am using cyclin E (B-7): sc-48420 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: DefinitelyNotMatt
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 4 out of 5 by from Reasonably specific and sensitive in WB with K Reasonably specific and sensitive in WB with K-562 and Jurkat nuclear extracts. -SCBT QC
Date published: 2014-02-01
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