Anti-cyclin D3 Antibody (DCS-22) recommended for detection of cyclin D3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-cyclin D3 Antibody (DCS-22): sc-56307

Anti-cyclin D3 Antibody (DCS-22) is rated 4.8 out of 5 by 4.
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Datasheets

    • cyclin D3 Antibody (DCS-22) is a mouse monoclonal IgG1 κ, cited in 8 publications, provided at 200 µg/ml
    • raised against full length cyclin D3 of human origin
    • Anti-cyclin D3 Antibody (DCS-22) is recommended for detection of cyclin D3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-cyclin D3 Antibody (DCS-22) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin D3 (DCS-22): sc-56307.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for cyclin D3 Antibody (DCS-22) for WB and IHC(P) applications. These reagents are now offered in bundles with cyclin D3 Antibody (DCS-22) (see ordering information below).
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I am using cyclin D3 (DCS-22): sc-56307 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Randy McDonald
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Specific Western detection in rat INS-1 cells 0 ug loads of rat insulinoma (INS-1) lysates were probed overnight and returned a strong, specific band of ~38 kD. The attached picture is a triplicate set (identical lanes)
Date published: 2019-05-22
Rated 4 out of 5 by from a relative good antibody for WB This antibody generates a signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract), although the signal intensity is not strong. I would recommend other products (e.g. sc-453 or sc--6283) for WB
Date published: 2017-09-18
Rated 5 out of 5 by from Clean blot with single strong band in C6 Clean blot with single strong band in C6, K-562, PMA treated K-562 and Jurkat whole cell lysates. -SCBT QC
Date published: 2015-03-26
Rated 5 out of 5 by from This product works great for IHC paraffin This product works great for IHC paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2014-06-19
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