cyclin D3 Antibody (D-7)recommended for detection of cyclin D3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

cyclin D3 Antibody (D-7): sc-6283

cyclin D3 Antibody (D-7) is rated 4.3 out of 5 by 17.
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Datasheets

    • cyclin D3 Antibody (D-7) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-292 representing full length cyclin D3 of human origin
    • cyclin D3 Antibody (D-7) is recommended for detection of cyclin D3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • cyclin D3 Antibody (D-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to TRITC for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin D3 (D-7): sc-6283.
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Can this mouse monoclonal antibody be used for immunohistochemical staining of mouse tissue?

Asked by: AbPolly
Thank you for your question. Yes, we recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody is available conjugated to HRP, PE, FITC, TRITC, AF488 and AF647.
Answered by: Technical Support 9
Date published: 2017-01-20
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Rated 1 out of 5 by from Non-specific Western detection in rat INS-1 lysate Tested by Western Blot (Li-Cor reagents) of rat insulinoma (INS-1) cell line. 60 ug loads were probed overnight, but only non-specific bands were detected. Recommend using sc-56307
Date published: 2019-05-22
Rated 4 out of 5 by from Worked well for WB worked well for WB using human tissue lysates at 1:200
Date published: 2018-03-22
Rated 5 out of 5 by from a good antibody for WB This antibody generates a clear signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 4 out of 5 by from Faint but distinct bands on WB Faint but distinct bands on WB. Used the antibody at 1:1000 dilution.
Date published: 2017-07-03
Rated 3 out of 5 by from not suitable for turbot there was no band in turbot, primary antibody 1:200, second antibody 1:2000. so i dont think it is suitable for turbot
Date published: 2017-06-20
Rated 5 out of 5 by from suitable for WB We use this antibody routinely by WB, it always gives very good signal,1:400 dilution .
Date published: 2017-06-03
Rated 5 out of 5 by from Good WB analysis We detected positive signal in WB using Hela cell,I highly recommend use 1:600 diluiton
Date published: 2017-05-28
Rated 3 out of 5 by from WB We have got many none specific bands in human macrophage lysate.
Date published: 2017-04-23
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