Anti-cyclin D3 Antibody (1) recommended for detection of cyclin D3 of mouse, rat, human and canine origin by WB, IP and IF

Anti-cyclin D3 Antibody (1): sc-135875

Anti-cyclin D3 Antibody (1) is rated 4.0 out of 5 by 1.
  • y_2021, m_8, d_5, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.18
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_en_US, sid_135875, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 110ms
  • REVIEWS, PRODUCT
loading
5
0
4
1
3
0
2
0
1
0

Datasheets

    • cyclin D3 Antibody (1) is a mouse monoclonal IgG2b, cited in 3 publications, provided at 50 µg/0.5 ml
    • raised against amino acids 127-292 of cyclin D3 of human origin
    • recommended for detection of cyclin D3 of mouse, rat, human and canine origin by WB, IP and IF
    • See cyclin D3 (D-7): sc-6283 for cyclin D3 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for cyclin D3 Antibody (1). This work is in progress.
loading

I am using cyclin D3 (1): sc-135875 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Professor Griffin
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
  • y_2021, m_8, d_5, h_19CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.18
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_135875, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 136ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Works pretty well for WB in RSV Works pretty well for WB in RSV-3T3 whole cell lysate. -SCBT QC
Date published: 2014-12-21
  • y_2021, m_8, d_5, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.18
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_en_US, sid_135875, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 14ms
  • REVIEWS, PRODUCT