Anti-cyclin D3 Antibody (18B6-10) recommended for detection of cyclin D3 p34 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-cyclin D3 Antibody (18B6-10): sc-453

Anti-cyclin D3 Antibody (18B6-10) is rated 5.0 out of 5 by 6.
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Datasheets

    • cyclin D3 Antibody (18B6-10) is a rat monoclonal IgG2a, cited in 39 publications, provided at 200 µg/ml
    • raised against recombinant cyclin D3 protein of mouse origin
    • Anti-cyclin D3 Antibody (18B6-10) is recommended for detection of cyclin D3 p34 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-cyclin D3 Antibody (18B6-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin D3 (18B6-10): sc-453.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for cyclin D3 Antibody (18B6-10). This work is in progress.
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Can cyclin D3 (18B6-10): sc-453 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: AbPolly
Thank you for your question. Yes, cyclin D3 (18B6-10): sc-453 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from great antibody for western I have tried this antibody on M14 and it comes out pretty well. A very clear band.
Date published: 2017-10-13
Rated 5 out of 5 by from a good antibody for WB This antibody generates a clear signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 5 out of 5 by from great features A very good products recommended to everyone It give me great help
Date published: 2016-11-26
Rated 5 out of 5 by from Great staining with paraffin Great staining with paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-02-12
Rated 5 out of 5 by from Good WB publishable data gathered with mouse Good WB publishable data gathered with mouse embryonic fibroblasts. -SCBT Publication Review
Date published: 2015-02-04
Rated 5 out of 5 by from Great antibody for WB Great antibody for WB, works in Jurkat cells. -SCBT QC
Date published: 2014-07-21
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