cyclin D2 Antibody (DCS-5) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
raised against full length cyclin D2 of human origin
cyclin D2 Antibody (DCS-5) is recommended for detection of cyclin D2 of mouse, rat and human origin by WB, IP, IF, FCM and ELISA
cyclin D2 Antibody (DCS-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin D2 (DCS-5): sc-53637.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
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Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
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Labware ships FedEx Ground free of charge to the contiguous US
The proliferation of eukaryotic cells is controlled at specific points in the cell cycle, particularly at the G1 to S and the G2 to M transitions. It is well established that the Cdc2 p34-cyclin B protein kinase plays a critical role in the G2 to M transition while cyclin A associates with Cdk2 p33 and functions in S phase. Considerable effort directed towards the identification of G1 cyclins has led to the isolation of cyclin D, cyclin C and cyclin E. Of these, cyclin D corresponds to a putative human oncogene, designated PRAD1, which maps at the site of the Bcl-1 rearrangement in certain lymphomas and leukemias. Two additional human type D cyclins, as well as their mouse homologs, have been identified. Evidence has established that members of the cyclin D family function to regulate phosphorylation of the retinoblastoma gene product, thereby activating E2F transcription factors.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Can cyclin D2 (DCS-5): sc-53637 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?
Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, cyclin D2 (DCS-5): sc-53637 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Rated 5 out of
Specific Western detection in rat INS-1 cells60 ug loads of rat insulinoma (INS-1) lysates were probed overnight and returned a strong, specific band of ~38 kD. Antibody sc-56305 is also suitable. The attached picture is a triplicate set (identical lanes)
Date published: 2019-05-22
Rated 5 out of
Nice signal by WB on mouse colon extractsThis antibody works perfectly on mouse colon extracts (20 ug) at a 1/200 dilution with regular ECL.
Date published: 2017-10-27
Rated 4 out of
a relative good antibody for WBThis antibody generates a signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract). However, I would recommend to use sc-376676 for WB.
Date published: 2017-09-18
Rated 3 out of
not very cleanIn HeLa, it is a low-affinity antibody. Not very clean, it has many nonspecific larger MW bands which are stronger than the cyclin-D2 band.
Date published: 2017-04-12
Rated 5 out of
Mono works in human transfected lysatesMono works in human transfected lysates. -SCBT QC
Date published: 2015-02-12
Rated 5 out of
Works well in MMWorks well in MM-142 nuclear extract under reducing and non-reducing conditions. -SCBT QC
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