Anti-cyclin D2 Antibody (DCS-3) recommended for detection of cyclin D2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-cyclin D2 Antibody (DCS-3): sc-56305

Anti-cyclin D2 Antibody (DCS-3) is rated 5.0 out of 5 by 4.
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Datasheets

    • cyclin D2 Antibody (DCS-3) is a mouse monoclonal IgG2a κ, cited in 7 publications, provided at 200 µg/ml
    • raised against full length cyclin D2 of human origin
    • Anti-cyclin D2 Antibody (DCS-3) is recommended for detection of cyclin D2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-cyclin D2 Antibody (DCS-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin D2 (DCS-3): sc-56305.
    • m-IgG Fc BP-HRP is the preferred secondary detection reagent for cyclin D2 Antibody (DCS-3) for WB and IHC(P) applications. This reagent is now offered in a bundle with cyclin D2 Antibody (DCS-3) (see ordering information below).
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I am using cyclin D2 (DCS-3): sc-56305 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Specific Western detection in rat INS-1 cells 60 ug loads of rat insulinoma (INS-1) lysates were probed overnight and returned a specific, moderately strong band of ~38 kD. Can also use sc-53637. The attached picture is a triplicate set (identical lanes)
Date published: 2019-05-22
Rated 5 out of 5 by from Popular choice for WB! The (DCS-3) clone is a popular choice for Western blotting. It has been featured in at least four publications in the literature for this application.
Date published: 2017-02-03
Rated 5 out of 5 by from Excellent antibody for WB with MM Excellent antibody for WB with MM-142. -SCBT QC
Date published: 2015-02-12
Rated 5 out of 5 by from Robust staining in paraffin Robust staining in paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2014-12-17
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