Anti-cyclin D2 Antibody (A-1) recommended for detection of cyclin D2 of human origin by WB, IP, IF and ELISA

Anti-cyclin D2 Antibody (A-1): sc-166288

Anti-cyclin D2 Antibody (A-1) is rated 3.0 out of 5 by 1.
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Datasheets

    • cyclin D2 Antibody (A-1) is a mouse monoclonal IgG2b, cited in 1 publications, provided at 200 µg/ml
    • raised against amino acids 1-289 representing full length cyclin D2 of human origin
    • recommended for detection of cyclin D2 of human origin by WB, IP, IF and ELISA
    • See cyclin D2 (B-6): sc-376676 for cyclin D2 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG2b BP-HRP is the preferred secondary detection reagent for cyclin D2 Antibody (A-1) for WB applications. This reagent is now offered in a bundle with cyclin D2 Antibody (A-1) (see ordering information below).
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I am using cyclin D2 (A-1): sc-166288 monoclonal antibody for immunofluorescence, what dilution of this antibody should I use?

Asked by: AbPolly
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-04-05
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Rated 3 out of 5 by from Works well in human transfected lysates Works well in human transfected lysates. -SCBT QC
Date published: 2013-06-24
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