Anti-cyclin D2 Antibody (34B1-3) recommended for detection of cyclin D2 and, to a lesser extent, cyclin D1 of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-cyclin D2 Antibody (34B1-3): sc-452

Anti-cyclin D2 Antibody (34B1-3) is rated 4.3 out of 5 by 9.
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Datasheets

    • cyclin D2 Antibody (34B1-3) is a rat monoclonal IgG2a, cited in 81 publications, provided at 200 µg/ml
    • raised against recombinant cyclin D2 protein
    • Anti-cyclin D2 Antibody (34B1-3) is recommended for detection of cyclin D2 and, to a lesser extent, cyclin D1 of mouse, rat and human origin by WB, IP, IF and IHC(P); non cross-reactive with cyclin D3
    • Anti-cyclin D2 Antibody (34B1-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to TRITC for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin D2 (34B1-3): sc-452.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for cyclin D2 Antibody (34B1-3). This work is in progress.
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Can cyclin D2 (34B1-3): sc-452 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Trav11
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, cyclin D2 (34B1-3): sc-452 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Detects cyclin D2 Detects human cyclin D2 in transfected COS cells by immunoblot.
Date published: 2019-05-14
Rated 1 out of 5 by from Didnot work for WB Did not work for western - samples were human cells in culture
Date published: 2017-11-21
Rated 5 out of 5 by from a good antibody for WB This antibody generates a clear signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 4 out of 5 by from Use this antibody routinely in WB in human cells Use this antibody routinely in WB in human cells.
Date published: 2015-07-29
Rated 4 out of 5 by from no comment no comment
Date published: 2015-04-29
Rated 5 out of 5 by from Works for WB in human A549 and mouse NIH Works for WB in human A549 and mouse NIH 3T3 samples. -SCBT Publication Review
Date published: 2015-02-12
Rated 5 out of 5 by from Works very nicely for IF on HeLa samples Works very nicely for IF on HeLa samples. -SCBT QC
Date published: 2015-01-22
Rated 5 out of 5 by from Great antibody for WB and IP with human heart Great antibody for WB and IP with human heart samples. -SCBT Publication Review
Date published: 2015-01-08
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