Anti-cyclin D1 Antibody (HD11) recommended for detection of cyclin D1 of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-cyclin D1 Antibody (HD11): sc-246

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Anti-cyclin D1 Antibody (HD11) is rated 4.38 out of 5 by 16.
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Datasheets

    • cyclin D1 Antibody (HD11) is a mouse monoclonal IgG1, cited in 391 publications, provided at 200 µg/ml
    • raised against recombinant cyclin D1 of human origin
    • Anti-cyclin D1 Antibody (HD11) is recommended for detection of cyclin D1 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • Anti-cyclin D1 Antibody (HD11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin D1 (HD11): sc-246.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for cyclin D1 Antibody (HD11). This work is in progress.
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Hi, I ve just used the antibody sc-246 for mouse tissue and I am getting two different bands. One at around 50kDa and another one at around 25kDa. Can I accept one of these as mouse Cyclin D1 ? Thank you in advance.

Asked by: ElliPapas
Dear Ellipapas, Thank you for your question. We want you to be successful in your research. To help you, i need more Information about your Protocol. Please contact our European Support office by phone: toll-free 00800 4573 8000, email [email protected] or live chat. We are happy to help you to troubleshoot the experiment.
Answered by: Technical Support Europe
Date published: 2017-08-15

I would like to use the cyclin D1 Antibody (HD11): sc-246 for IHC(P) in mouse tissue. Is this possible?

Asked by: cjMara
Thank you for your question. If looking to use a mouse monoclonal primary antibody for IHC(P) in mouse tissue, we recommend using our directly conjugated primary antibodies. Currently, sc-246 is not available with direct conjugation, but an alternative cyclin D1 antibody, sc-8396, is available with conjugation to agarose, HRP, FITC, PE, Alexa Fluor 488 and Alexa Fluor 647. If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 15
Date published: 2017-01-22

I would like to use the cyclin D1 Antibody (HD11): sc-246 for IHC(P) in mouse tissue. Is this possible?

Asked by: Trav11
Thank you for your question. If looking to use a mouse monoclonal primary antibody for IHC(P) in mouse tissue, we recommend using our directly conjugated primary antibodies. Currently, sc-246 is not available with direct conjugation, but an alternative cyclin D1 antibody, sc-8396, is available with conjugation to agarose, HRP, FITC, PE, Alexa Fluor 488 and Alexa Fluor 647. If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Service 11
Date published: 2017-01-16
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