cyclin D1 Antibody (HD11)recommended for detection of cyclin D1 of mouse, rat and human origin by WB, IP, IF and IHC(P)

cyclin D1 Antibody (HD11): sc-246

cyclin D1 Antibody (HD11) is rated 4.3 out of 5 by 15.
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Datasheets

    • cyclin D1 Antibody (HD11) is a mouse monoclonal IgG1 provided at 200 µg/ml
    • raised against recombinant cyclin D1 of human origin
    • cyclin D1 Antibody (HD11) is recommended for detection of cyclin D1 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • cyclin D1 Antibody (HD11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin D1 (HD11): sc-246.
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Hi, I ve just used the antibody sc-246 for mouse tissue and I am getting two different bands. One at around 50kDa and another one at around 25kDa. Can I accept one of these as mouse Cyclin D1 ? Thank you in advance.

Asked by: ElliPapas
Dear Ellipapas, Thank you for your question. We want you to be successful in your research. To help you, i need more Information about your Protocol. Please contact our European Support office by phone: toll-free 00800 4573 8000, email europe@scbt.com or live chat. We are happy to help you to troubleshoot the experiment.
Answered by: Technical Support Europe
Date published: 2017-08-15

I would like to use the cyclin D1 Antibody (HD11): sc-246 for IHC(P) in mouse tissue. Is this possible?

Asked by: cjMara
Thank you for your question. If looking to use a mouse monoclonal primary antibody for IHC(P) in mouse tissue, we recommend using our directly conjugated primary antibodies. Currently, sc-246 is not available with direct conjugation, but an alternative cyclin D1 antibody, sc-8396, is available with conjugation to agarose, HRP, FITC, PE, Alexa Fluor 488 and Alexa Fluor 647. If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 15
Date published: 2017-01-22

I would like to use the cyclin D1 Antibody (HD11): sc-246 for IHC(P) in mouse tissue. Is this possible?

Asked by: Trav11
Thank you for your question. If looking to use a mouse monoclonal primary antibody for IHC(P) in mouse tissue, we recommend using our directly conjugated primary antibodies. Currently, sc-246 is not available with direct conjugation, but an alternative cyclin D1 antibody, sc-8396, is available with conjugation to agarose, HRP, FITC, PE, Alexa Fluor 488 and Alexa Fluor 647. If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Service 11
Date published: 2017-01-16
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Rated 1 out of 5 by from No Western signal in INS-1 rat cell lysates Tested by Western Blot (Li-Cor reagents) of rat insulinoma (INS-1) cell line. 60 ug loads were probed overnight, but no bands were detected. Recommend sc-20044 instead
Date published: 2019-05-22
Rated 4 out of 5 by from weak signal very weak signal on Hela cell lysate with some background
Date published: 2018-08-22
Rated 4 out of 5 by from GOOD ANTIBODY I got a sample of this sample as a free sample and it is working quite good. I have plans to buy this antibody as soon as possible
Date published: 2018-03-29
Rated 4 out of 5 by from Specific binding I bought this 5 years back during my PhD program for our Molecular Medicine laboratory in India. This antibody has good specificity to cell lysates of human origin. It worked well with a dilution of 1:2500 and the bands were clearly visible through ECL detection even after 3 to 4 reuses of the diluted antibody.
Date published: 2017-06-22
Rated 4 out of 5 by from Good single band Good clear band. Little to no background. Specificity on point!
Date published: 2017-06-07
Rated 5 out of 5 by from Used in IHC Worked well at 1:100 dilution for IHC on rat brain sections, very happy.
Date published: 2017-04-07
Rated 5 out of 5 by from Strong western data Very specific western blot results; strong clear signal
Date published: 2017-01-30
Rated 5 out of 5 by from Strong cyclin D1 clone Extremely well-published cyclin D1 antibody suitable for mouse, rat, and human detection. Well-established, great option for cyclin D1 detection.
Date published: 2017-01-24
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