Anti-cyclin D1 Antibody (CD1.1) recommended for detection of cyclin D1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-cyclin D1 Antibody (CD1.1): sc-56302

Anti-cyclin D1 Antibody (CD1.1) is rated 4.0 out of 5 by 2.
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Datasheets

    • cyclin D1 Antibody (CD1.1) is a mouse monoclonal IgG1, cited in 23 publications, provided at 50 µg/0.5 ml
    • raised against full length cyclin D1 of human origin
    • recommended for detection of cyclin D1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See cyclin D1 (A-12): sc-8396 for cyclin D1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for cyclin D1 Antibody (CD1.1). This work is in progress.
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Can sc-56302: cyclin D1 (CD1.1) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Randy McDonald
Thank you for your question. Yes, sc-56302: cyclin D1 (CD1.1) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Band at expected mw but a little messy in RAW 264 Band at expected mw but a little messy in RAW 264.7 whole cell lysate. -SCBT QC
Date published: 2015-02-26
Rated 4 out of 5 by from Band at expected size Band at expected size, a little faint in C32, KNRK and MCF7 nuclear extracts and C6 whole cell lysate. -SCBT QC
Date published: 2014-05-15
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