cyclin D1 Antibody (A-12)recommended for detection of cyclin D1 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA

cyclin D1 Antibody (A-12): sc-8396

cyclin D1 Antibody (A-12) is rated 4.3 out of 5 by 66.
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Datasheets

    • cyclin D1 Antibody (A-12) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-295 representing full length cyclin D1 of human origin
    • cyclin D1 Antibody (A-12) is recommended for detection of cyclin D1 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA; may cross-react with cyclin D2; non cross-reactive with cyclin D3
    • cyclin D1 Antibody (A-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to either TRITC or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin D1 (A-12): sc-8396.
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What is the recommended secondary detection reagent for cyclin D1 Antibody (A-12) in Western blot applications?

Asked by: Trav11
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 8
Date published: 2016-12-29
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Rated 3 out of 5 by from Decent Western detection in rat INS-1 cells 60 ug loads of rat insulinoma (INS-1) lysates were probed overnight and returned a strong band of ~38 kD and several non-specific bands. Recommend using sc-20044 instead. The attached picture is a triplicate set (identical lanes)
Date published: 2019-05-22
Rated 2 out of 5 by from Not really work for IF I used 1:20 dilution for IF staining of chondrocytes on cryo sections, but it does not really work. The one that I used is conjugated to Alexa 647.
Date published: 2019-05-06
Rated 4 out of 5 by from Working for WB It works for dettecting mouse and human protein by western blot
Date published: 2019-01-17
Rated 3 out of 5 by from Cyclin D1 western blot We use Cyclin D1 antibody to study proliferation and cell cycle of human amniotic stem cells during their hepatic differentiation. It is an excellent antibody for western blot in human samples. Nevertheless, we have to say that sometimes the weight of the protein does not match the weight of the band.
Date published: 2018-05-17
Rated 5 out of 5 by from UPENN Gα 12 Antibody is very good. The band is very stronger.
Date published: 2018-04-19
Rated 5 out of 5 by from Worked Great!! Worked great for human and mouse tissue samples when probing at 1:200 via WB. Highly recommend!
Date published: 2018-03-22
Rated 4 out of 5 by from Good antibody The bands were at the expected size and they were nicely visible.
Date published: 2018-03-14
Rated 5 out of 5 by from WB I got clear bands and correct position by wextern blot (1:200).
Date published: 2018-03-01
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