cyclin D1 Antibody (A-12) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
raised against amino acids 1-295 representing full length cyclin D1 of human origin
cyclin D1 Antibody (A-12) is recommended for detection of cyclin D1 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA; may cross-react with cyclin D2; non cross-reactive with cyclin D3
cyclin D1 Antibody (A-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to either TRITC or Alexa Fluor® 405 for IF, IHC(P) and FCM
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin D1 (A-12): sc-8396.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
The proliferation of eukaryotic cells is controlled at specific points in the cell cycle, particularly at the G1 to S and the G2 to M transitions. It is well established that the Cdc2 p34-cyclin B protein kinase plays a critical role in the G2 to M transition while cyclin A associates with Cdk2 p33 and functions in S phase. Considerable effort directed towards the identification of G1 cyclins has led to the isolation of cyclin D, cyclin C and cyclin E. Of these, cyclin D corresponds to a putative human oncogene, designated PRAD1, which maps at the site of the Bcl-1 rearrangement in certain lymphomas and leukemias. Two additional human type D cyclins, as well as their mouse homologs, have been identified. Evidence has established that members of the cyclin D family function to regulate phosphorylation of the retinoblastoma gene product, thereby activating E2F transcription factors.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What is the recommended secondary detection reagent for cyclin D1 Antibody (A-12) in Western blot applications?
Asked by: Trav11
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here:
Rated 3 out of
Decent Western detection in rat INS-1 cells60 ug loads of rat insulinoma (INS-1) lysates were probed overnight and returned a strong band of ~38 kD and several non-specific bands. Recommend using sc-20044 instead. The attached picture is a triplicate set (identical lanes)
Date published: 2019-05-22
Rated 2 out of
MDee Ng from
Not really work for IFI used 1:20 dilution for IF staining of chondrocytes on cryo sections, but it does not really work. The one that I used is conjugated to Alexa 647.
Date published: 2019-05-06
Rated 4 out of
Working for WBIt works for dettecting mouse and human protein by western blot
Date published: 2019-01-17
Rated 3 out of
Cyclin D1 western blotWe use Cyclin D1 antibody to study proliferation and cell cycle of human amniotic stem cells during their hepatic differentiation. It is an excellent antibody for western blot in human samples. Nevertheless, we have to say that sometimes the weight of the protein does not match the weight of the band.
Date published: 2018-05-17
Rated 5 out of
UPENNGα 12 Antibody is very good. The band is very stronger.
Date published: 2018-04-19
Rated 5 out of
Worked Great!!Worked great for human and mouse tissue samples when probing at 1:200 via WB. Highly recommend!
Date published: 2018-03-22
Rated 4 out of
Good antibodyThe bands were at the expected size and they were nicely visible.
Date published: 2018-03-14
Rated 5 out of
WBI got clear bands and correct position by wextern blot (1：200).
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn
Create a new account
Email address already exists, please enter a new valid email address.Hide
USE YOUR SOCIAL NETWORK
Create an account quickly and easily with your preferred social network account. You won't have to remember an extra name and password.
Creating an account with us makes your shopping experience much easier and faster. You can save favorites, save cart, check order status and speed through checkout with saved addresses, payment methods and more.