cyclin D1 Antibody (72-13G) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
raised against recombinant fusion protein of mouse origin
cyclin D1 Antibody (72-13G) is recommended for detection of cyclin D1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and KA; may cross-react with cyclin D2 or cyclin D3 encoded proteins
cyclin D1 Antibody (72-13G) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin D1 (72-13G): sc-450.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
The proliferation of eukaryotic cells is controlled at specific points in the cell cycle, particularly at the G1 to S and the G2 to M transitions. It is well established that the Cdc2 p34-cyclin B protein kinase plays a critical role in the G2 to M transition while cyclin A associates with Cdk2 p33 and functions in S phase. Considerable effort directed towards the identification of G1 cyclins has led to the isolation of cyclin D, cyclin C and cyclin E. Of these, cyclin D corresponds to a putative human oncogene, designated PRAD1, which maps at the site of the Bcl-1 rearrangement in certain lymphomas and leukemias. Two additional human type D cyclins, as well as their mouse homologs, have been identified. Evidence has established that members of the cyclin D family function to regulate phosphorylation of the retinoblastoma gene product, thereby activating E2F transcription factors.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
I am wondering about the epitope for the sc-450 antibody.
Asked by: Masha Brooun
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2019-04-11
Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?
Asked by: Anna99
While this antibody is recommended for IHC, best results of mouse monoclonal antibody staining on mouse tissue, come from directly conjugated antibodies. See cyclin D1 (A-12): sc-8396 for cyclin D1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647.
Rated 2 out of
Weak Western detection in rat INS-1 lysates60 ug loads of rat insulinoma (INS-1) lysates were probed overnight and returned a faint band of ~38 kD. Recommend using sc-20044 instead. The attached picture is a triplicate set (identical lanes)
Date published: 2019-05-22
Rated 1 out of
no band in my membraneno band in my membrane. the service after sold of santa cruz is more perfect than other compay suh as abcam and CST which there are a lot of requirment if you want to return the product.
Date published: 2018-04-22
Rated 4 out of
a good antibody for WBThis antibody generates a signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract). although the intensity is not very strong. For WB, I would recommend other products such as sc-8396 or sc-20044.
Date published: 2017-09-18
Rated 3 out of
this antibody can be used in WB.I use it in WB . SK-N-SH cell lysis used。 10% gel，electrophoresis： 30 mA,90 min。 transfer membrane ：300 ma，45min。5% milk block，1：:100 antibody overnight。1X TTBS wash 3 times ，second antibody 1:2000 ，1 h ， temperature。then 1X TTBS wash 3 times。 15 min can appearance bands sometimes。but there is a specific band。
Date published: 2017-08-31
Rated 5 out of
work wellcyclin D1 work well for wb. It suitable for detect endogenous cyclin D1 in primary murine macrophages as it should according to datasheet by WB. It got satisfied band.
Date published: 2017-08-19
Rated 5 out of
cyclin D1 antibodyThis cyclin D1 antibody seems more better than the polyclonal antibody sc-753 which I used before.It's more specific and easy to detect cyclin D1,I prefer the dilution 1:1000.
Date published: 2017-06-25
Rated 5 out of
really goodVery good signal in mice intestine for western blot, I am happy with the results
Date published: 2017-06-01
Rated 5 out of
Great choiceExcellent nuclear signal seen in IF and IHC in different samples.
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn
Create a new account
Email address already exists, please enter a new valid email address.Hide
USE YOUR SOCIAL NETWORK
Create an account quickly and easily with your preferred social network account. You won't have to remember an extra name and password.
Creating an account with us makes your shopping experience much easier and faster. You can save favorites, save cart, check order status and speed through checkout with saved addresses, payment methods and more.