cyclin D1 Antibody (72-13G)recommended for detection of cyclin D1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and KA

cyclin D1 Antibody (72-13G): sc-450

cyclin D1 Antibody (72-13G) is rated 4.4 out of 5 by 17.
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Datasheets

    • cyclin D1 Antibody (72-13G) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against recombinant fusion protein of mouse origin
    • cyclin D1 Antibody (72-13G) is recommended for detection of cyclin D1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and KA; may cross-react with cyclin D2 or cyclin D3 encoded proteins
    • cyclin D1 Antibody (72-13G) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin D1 (72-13G): sc-450.
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Hello, I am wondering about the epitope for the sc-450 antibody. thank you. Masha

Asked by: Masha Brooun
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2019-04-11

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: Anna99
While this antibody is recommended for IHC, best results of mouse monoclonal antibody staining on mouse tissue, come from directly conjugated antibodies. See cyclin D1 (A-12): sc-8396 for cyclin D1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647.
Answered by: Tech Service 5
Date published: 2017-01-05
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Rated 2 out of 5 by from Weak Western detection in rat INS-1 lysates 60 ug loads of rat insulinoma (INS-1) lysates were probed overnight and returned a faint band of ~38 kD. Recommend using sc-20044 instead. The attached picture is a triplicate set (identical lanes)
Date published: 2019-05-22
Rated 1 out of 5 by from no band in my membrane no band in my membrane. the service after sold of santa cruz is more perfect than other compay suh as abcam and CST which there are a lot of requirment if you want to return the product.
Date published: 2018-04-22
Rated 4 out of 5 by from a good antibody for WB This antibody generates a signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract). although the intensity is not very strong. For WB, I would recommend other products such as sc-8396 or sc-20044.
Date published: 2017-09-18
Rated 3 out of 5 by from this antibody can be used in WB. I use it in WB . SK-N-SH cell lysis used。 10% gel,electrophoresis: 30 mA,90 min。 transfer membrane :300 ma,45min。5% milk block,1::100 antibody overnight。1X TTBS wash 3 times ,second antibody 1:2000 ,1 h , temperature。then 1X TTBS wash 3 times。 15 min can appearance bands sometimes。but there is a specific band。
Date published: 2017-08-31
Rated 5 out of 5 by from work well cyclin D1 work well for wb. It suitable for detect endogenous cyclin D1 in primary murine macrophages as it should according to datasheet by WB. It got satisfied band.
Date published: 2017-08-19
Rated 5 out of 5 by from cyclin D1 antibody This cyclin D1 antibody seems more better than the polyclonal antibody sc-753 which I used before.It's more specific and easy to detect cyclin D1,I prefer the dilution 1:1000.
Date published: 2017-06-25
Rated 5 out of 5 by from really good Very good signal in mice intestine for western blot, I am happy with the results
Date published: 2017-06-01
Rated 5 out of 5 by from Great choice Excellent nuclear signal seen in IF and IHC in different samples.
Date published: 2017-01-11
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