Anti-cyclin B2 Antibody (A-2) recommended for detection of cyclin B2 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-cyclin B2 Antibody (A-2): sc-28303

Anti-cyclin B2 Antibody (A-2) is rated 4.4 out of 5 by 14.
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Datasheets

    • Anti-cyclin B2 Antibody (A-2) is a mouse monoclonal IgG1 κ cyclin B2 antibody, cited in 15 publications, provided at 200 µg/ml
    • raised against amino acids 1-105 of cyclin B2 of human origin
    • Anti-cyclin B2 Antibody (A-2) is recommended for detection of cyclin B2 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-cyclin B2 Antibody (A-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin B2 (A-2): sc-28303.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for cyclin B2 Antibody (A-2) for WB applications. These reagents are now offered in bundles with cyclin B2 Antibody (A-2) (see ordering information below).
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Are there any issues with using sc-28303: cyclin B2 (A-2) mouse monoclonal primary antibody on mouse samples?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-28303 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 3 out of 5 by from Just works for WB Loading amount: 10 ug of human MPNST (ST8814) cells Substrate: SuperSignal™ West Femto Maximum Sensitivity Substrate Dilution: 1:200 This antibody only worked with the most sensitive substrate.
Date published: 2019-11-26
Rated 1 out of 5 by from No Western signal in INS-1 rat cell lysates Tested by Western Blot (Li-Cor reagents) of rat insulinoma (INS-1) cell line. 60 ug loads were probed overnight, but no bands were detected. Recommend using CcnB1 antibody sc-7393 instead
Date published: 2019-05-22
Rated 5 out of 5 by from Works perfectly by WB on mouse colon extracts I tried this antobody on mouse colon protein extracts at a 1/200 dilution with around 20 ug of protein quantity. It showed a very nice signal at the right molecular weight
Date published: 2017-10-27
Rated 5 out of 5 by from 抗体效果不错 今年年初,申请了该抗体的单克隆试用装,效果不错。组织标本做的WB,条带的特异性很好,首次使用,背景不高,但不可回收再利用。
Date published: 2017-09-25
Rated 5 out of 5 by from a good antibody for WB This antibody generates a clear signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 4 out of 5 by from not suitable for turbot there's no band in turbot. I used 1:100 and 1:2000 for second antibody ,exposed 10mns, no band
Date published: 2017-06-06
Rated 4 out of 5 by from 没法在大菱鲆上使用 1:100用于大菱鲆细胞,曝了很长时间才有很浅很浅的带,杂带也较多,不太适合于大菱鲆。但是在3T3细胞较好,条带清晰
Date published: 2017-06-05
Rated 5 out of 5 by from very good for WB We are very satisfied with cyclin B2 is really good and I have used it on my recent research, very good WB, signal strong at 1:500 dilution.
Date published: 2017-05-28
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