cyclin B1 Antibody (V92.1)recommended for detection of cyclin B1 of mouse, rat, human and hamster origin by WB and IP

cyclin B1 Antibody (V92.1): sc-53238

cyclin B1 Antibody (V92.1) is rated 4.0 out of 5 by 2.
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Datasheets

    • cyclin B1 Antibody (V92.1) is a mouse monoclonal mouse IgG1 κ (kappa light chain) provided at 200 µg/ml
    • raised against cyclin B1 of hamster orign
    • recommended for detection of cyclin B1 of mouse, rat, human and hamster origin by WB and IP
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See cyclin B1 (GNS1): sc-245 for cyclin B1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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What blocking solution should I use with cyclin B1 (V92.1): sc-53238 monoclonal antibody for Western blot?

Asked by: TinTin
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 3 out of 5 by from WB: Bands a little faint in K WB: Bands a little faint in K-562, PMA treated K-562, Jurkat and PMA induced Jurkat nuclear extracts. -SCBT QC
Date published: 2015-02-04
Rated 5 out of 5 by from Good clean WB with K Good clean WB with K-562. -SCBT QC
Date published: 2014-01-20
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