Anti-cyclin B1 Antibody (V152) recommended for detection of cyclin B1 of mouse, rat, human and hamster origin by WB, IP, IF, IHC(P) and FCM

Anti-cyclin B1 Antibody (V152): sc-53236

Anti-cyclin B1 Antibody (V152) is rated 4.5 out of 5 by 4.
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Datasheets

    • cyclin B1 Antibody (V152) is a mouse monoclonal IgG1 κ, cited in 10 publications, provided at 200 µg/ml
    • raised against His-tagged recombinant cyclin B1 of hamster origin
    • recommended for detection of cyclin B1 of mouse, rat, human and hamster origin by WB, IP, IF, IHC(P) and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See cyclin B1 (GNS1): sc-245 for cyclin B1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for cyclin B1 Antibody (V152) for WB and IHC(P) applications. These reagents are now offered in bundles with cyclin B1 Antibody (V152) (see ordering information below).
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Can cyclin B1 (V152): sc-53236 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, cyclin B1 (V152): sc-53236 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Perfect experiment This antibody is great, specific, clean and strong results by WB
Date published: 2017-08-25
Rated 5 out of 5 by from Good antibody for IF Good antibody for IF staining in human colon tissue
Date published: 2017-04-18
Rated 4 out of 5 by from Reasonably specific and sensitive Reasonably specific and sensitive, good results in WB with HeLa, PMA treated HeLa, K-562, PMA treated K-562, Jurkat and PMA treated Jurkat nuclear extracts. -SCBT QC
Date published: 2015-03-19
Rated 4 out of 5 by from Good antibody for Western Good antibody for Western, publication with epithelial ovarian cancer cell lines OVCA420, OVCA432, OVCA433, SKOV3, and HEY. -SCBT Publication Review
Date published: 2014-12-14
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