cyclin B1 Antibody (V152)recommended for detection of cyclin B1 of mouse, rat, human and hamster origin by WB, IP, IF, IHC(P) and FCM

cyclin B1 Antibody (V152): sc-53236

cyclin B1 Antibody (V152) is rated 4.5 out of 5 by 4.
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Datasheets

    • cyclin B1 Antibody (V152) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against His-tagged recombinant cyclin B1 of hamster origin
    • recommended for detection of cyclin B1 of mouse, rat, human and hamster origin by WB, IP, IF, IHC(P) and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See cyclin B1 (GNS1): sc-245 for cyclin B1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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Can cyclin B1 (V152): sc-53236 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, cyclin B1 (V152): sc-53236 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Perfect experiment This antibody is great, specific, clean and strong results by WB
Date published: 2017-08-25
Rated 5 out of 5 by from Good antibody for IF Good antibody for IF staining in human colon tissue
Date published: 2017-04-18
Rated 4 out of 5 by from Reasonably specific and sensitive Reasonably specific and sensitive, good results in WB with HeLa, PMA treated HeLa, K-562, PMA treated K-562, Jurkat and PMA treated Jurkat nuclear extracts. -SCBT QC
Date published: 2015-03-19
Rated 4 out of 5 by from Good antibody for Western Good antibody for Western, publication with epithelial ovarian cancer cell lines OVCA420, OVCA432, OVCA433, SKOV3, and HEY. -SCBT Publication Review
Date published: 2014-12-14
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