cyclin B1 Antibody (GNS1)recommended for detection of cyclin B1 of mouse, rat and human origin by WB, IP, IF and IHC(P)

cyclin B1 Antibody (GNS1): sc-245

cyclin B1 Antibody (GNS1) is rated 4.6 out of 5 by 40.
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Datasheets

    • cyclin B1 Antibody (GNS1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against a recombinant protein corresponding to human cyclin B1
    • cyclin B1 Antibody (GNS1) is recommended for detection of cyclin B1 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • cyclin B1 Antibody (GNS1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to either TRITC or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • TransCruz reagent for ChIP application (sc-245 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin B1 (GNS1): sc-245.
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Will this antibody react with bovine protein? Than you.

Asked by: clvleal
Thank you for your question. We are aware of one publication citing use of this antibody in bovine ABAE cells. Please refer to this reference on NCBI's PubMed, PMID: 15746189.
Answered by: Technical Support
Date published: 2017-08-02

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Sweetums
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 4
Date published: 2017-11-03

I need to use an antibody to detect the protein in samples of (other species, e.g., bovine, porcine, canine, equine, avian, non-mammalian) origin. Do you know if this mouse monoclonal antibody will work?

Asked by: Sweetums
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 4
Date published: 2016-12-16
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Rated 4 out of 5 by from Good for western blot The antibody can detect a specific band with 1:250 dilution for western blot. Samples were HeLa cell extract.
Date published: 2019-10-08
Rated 5 out of 5 by from anti-cyclin B1 antibody works for western blot. We used the anti-cyclin B1 antibody for western blotting of HeLa whole cell lysate and detected a band of the correct size.
Date published: 2019-07-29
Rated 1 out of 5 by from No Western signal in INS-1 rat cell lysates Tested by Western Blot (Li-Cor reagents) of rat insulinoma (INS-1) cell line. 60 ug loads were probed overnight, but no bands were detected. Alternatively, faint bands detected using sc-7393
Date published: 2019-05-22
Rated 5 out of 5 by from Figure 5-D Protein analysis by Western blot analysis of CCNB1 in three mutant lung adenocarcinoma cells (H1792.604, H2009, H358)
Date published: 2019-02-22
Rated 5 out of 5 by from wonderful antibody! 1:200 diluted for wb of rat protein ,the band is strong and special
Date published: 2018-06-12
Rated 5 out of 5 by from Good for WB This antibody is good for Western blot using human cell nuclear extract.
Date published: 2018-05-12
Rated 5 out of 5 by from Great product!! It's a great product for me to analyze Western blotting.
Date published: 2018-04-22
Rated 5 out of 5 by from 使用效果还不错 近期刚使用这个抗体,按照1:400的浓度测试,发出的条带符合说明书的介绍。清晰度之类符合预期,比较满意。
Date published: 2018-04-16
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