Anti-cyclin B1 Antibody (0.N.238) recommended for detection of cyclin B1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and KA

Anti-cyclin B1 Antibody (0.N.238): sc-70898

Anti-cyclin B1 Antibody (0.N.238) is rated 4.5 out of 5 by 2.
  • y_2021, m_10, d_19, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_70898, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 140ms
  • REVIEWS, PRODUCT
loading
5
1
4
1
3
0
2
0
1
0

Datasheets

    • cyclin B1 Antibody (0.N.238) is a mouse monoclonal IgG1 κ, cited in 15 publications, provided at 200 µg/ml
    • raised against a recombinant protein corresponding to human cyclin B1
    • recommended for detection of cyclin B1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and KA
    • See cyclin B1 (GNS1): sc-245 for cyclin B1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for cyclin B1 Antibody (0.N.238) for WB and IHC(P) applications. This reagent is now offered in a bundle with cyclin B1 Antibody (0.N.238) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
loading

How much sample do you recommend loading on the gel for Western blot detection using cyclin B1 (0.N.238): sc-70898?

Asked by: TinTin
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
  • y_2021, m_10, d_19, h_18CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_70898, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 129ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Band at expected size with transfected lysate Band at expected size with transfected lysate and K-562 nuclear extract -SCBT QC
Date published: 2015-01-30
Rated 5 out of 5 by from Published data in IF application with U2OS Published data in IF application with U2OS cell lines with and without the constitutive expression of Epstein-Barr virus (EBV) EBNA1 protein and polyomavirus large T antigen -SCBT Publication Review
Date published: 2013-01-22
  • y_2021, m_10, d_19, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_70898, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 18ms
  • REVIEWS, PRODUCT