cyclin B Antibody (D-1)recommended for detection of cyclin B of d origin by WB, IP, IF and ELISA

cyclin B Antibody (D-1): sc-166210

cyclin B Antibody (D-1) is rated 4.0 out of 5 by 8.
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Datasheets

    • cyclin B Antibody (D-1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-300 of cyclin B of Drosophila melanogaster origin
    • cyclin B Antibody (D-1) is recommended for detection of cyclin B of d origin by WB, IP, IF and ELISA
    • cyclin B Antibody (D-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin B (D-1): sc-166210.
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What blocking solution should I use with cyclin B (D-1): sc-166210 monoclonal antibody for Western blot?

Asked by: jerojero
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-04-13
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Rated 4 out of 5 by from Good features Good quality antibody for western blot detection of cyclin B in cell lysates with visible difference between proliferating and growth arrested cells
Date published: 2018-03-12
Rated 3 out of 5 by from an OK antibody for WB This antibody generates a signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract), albeit the intensity is somewhat weak
Date published: 2017-09-18
Rated 4 out of 5 by from not suitable for turbot there is a very light band when i exposed for 25min
Date published: 2017-07-10
Rated 1 out of 5 by from Didnot work for WB No band was observed in MCF-7 cell lysates. Used the ab at 1:1000 dilution.
Date published: 2017-07-03
Rated 5 out of 5 by from Great feature This antibody has been tested by WB for HepG2 cells. I used the product and diluted in TBST(1:200),4℃ overnight.
Date published: 2017-06-02
Rated 5 out of 5 by from show good result In my Western blot analysis,this cyclinB antibody show good performance.The band is very strong.
Date published: 2016-12-05
Rated 5 out of 5 by from It worked very nice on WB with Drosophila tissues It worked very nice on WB with Drosophila tissues.
Date published: 2015-05-19
Rated 5 out of 5 by from Great monoclonal antibody Great monoclonal antibody, good specificity and signal strength in Drosophila. -SCBT QC
Date published: 2014-11-23
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