cyclin A2 Antibody (46B11)recommended for detection of cyclin A2 of Xenopus laevis origin by WB

cyclin A2 Antibody (46B11): sc-53234

cyclin A2 Antibody (46B11) is rated 3.8 out of 5 by 4.
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Datasheets

    • cyclin A2 Antibody (46B11) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against SDS-derived malE-cyclin A2 fusion protein of Xenopus origin
    • cyclin A2 Antibody (46B11) is recommended for detection of cyclin A2 of Xenopus laevis origin by WB
    • cyclin A2 Antibody (46B11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin A2 (46B11): sc-53234.
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How much sample do you recommend loading on the gel for Western blot detection using cyclin A2 (46B11): sc-53234?

Asked by: DefinitelyNotMatt
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-27
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Rated 5 out of 5 by from Cyclin A2 (C19) Aplin lab used the Cyclin A2 (C19)antibody for western blotting.
Date published: 2019-02-06
Rated 5 out of 5 by from WB效果很好, 分子量在27左右,条带很清晰,牛奶封闭就可以。之前听师姐说不好用,就没定过,但是这次试了哈还不错,以后有需要就订这个了。
Date published: 2018-03-22
Rated 3 out of 5 by from needs more characterization This antibody generates multiple bands at the predicted position on the WB making it difficult to conclude whether it works or not (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
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