cyclin A Antibody (H-3)recommended for detection of cyclin A and cyclin A1 of mouse, rat and human origin by WB, IP, IF and ELISA

cyclin A Antibody (H-3): sc-271645

cyclin A Antibody (H-3) is rated 4.1 out of 5 by 9.
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Datasheets

    • cyclin A Antibody (H-3) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-432 representing full length cyclin A of human origin
    • cyclin A Antibody (H-3) is recommended for detection of cyclin A and cyclin A1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • cyclin A Antibody (H-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-271645 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin A (H-3): sc-271645.
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Can the X form of this antibody be used for applications other than ChIP and EMSA, such as Western Blot or IF?

Asked by: AbPolly
Thank you for your question. Yes, the X form of this antibody can be used in applications such as Western Blot or IF, the dilution factor just needs to be adjusted as it is ten times as concentrated as the standard form.
Answered by: Technical Support 15
Date published: 2017-01-26
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Rated 1 out of 5 by from No Western signal in INS-1 rat cell lysates Tested by Western Blot (Li-Cor reagents) of rat insulinoma (INS-1) cell line. 60 ug loads were probed overnight, but no bands were detected. Presence of CcnA protein in this cell line was previously confirmed using discontinued product sc-596
Date published: 2019-05-22
Rated 1 out of 5 by from no signal with HUVEC I got this sample 6 months ago to replace sc-596 for cyclin A with WB. With 35ug of HUVEC samples, I got NO signal after 5 minute exposure (primary 1:350, secondary 1:2000).
Date published: 2018-05-02
Rated 5 out of 5 by from Good ab for WB works well for western blotting with samples from human cultured cells
Date published: 2017-11-21
Rated 5 out of 5 by from Sehr gute ergebnisse WB 1-300 dilution ! I ordered 1 month ago, this cyclin a seems the best for mouse samples, I was working with primary cells isolated from young and old mice.
Date published: 2017-11-15
Rated 5 out of 5 by from a good antibody for WB This antibody generates a clear signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 5 out of 5 by from Nice bands Tested several samples with cyclin A Antibody (H-3) and observed strong and specific band
Date published: 2017-01-19
Rated 5 out of 5 by from Works well for IF in mouse (NIH3T3) Works well for IF in mouse (NIH3T3), publishable data. -SCBT Publication Review
Date published: 2015-03-05
Rated 5 out of 5 by from Clean blot Clean blot, publishable images with human WPE1, DU145, LNCaP, and PC3. -SCBT Publication Review
Date published: 2015-02-05
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